Quick Comparison

5-HTPHoly Basil (Tulsi)
Half-Life2-5 hours2-5 hours (eugenol and other active compounds)
Typical DosageStandard: 50-200 mg daily. For mood: 50-100 mg 2-3 times daily. For sleep: 100-300 mg 30-60 minutes before bed. Start low — some people are very sensitive. Take with food to reduce nausea.Standard: 300-600 mg extract daily, or 2-3 cups of tulsi tea. Standardized extracts (2.5% ursolic acid) provide more consistent dosing. Can be taken morning or evening.
AdministrationOral (capsules, tablets). Take with food to reduce GI side effects. Evening dosing preferred for sleep benefits.Oral (capsules, tea, tincture). Tea form is traditional and pleasant. Extract for standardized dosing.
Research Papers10 papers10 papers
Categories

Mechanism of Action

5-HTP

5-HTP readily crosses the blood-brain barrier via the large neutral amino acid transporter (LAT1/SLC7A5), unlike serotonin itself which cannot. Once in the brain, aromatic L-amino acid decarboxylase (AADC, also called DOPA decarboxylase) converts 5-HTP to serotonin (5-hydroxytryptamine) using pyridoxal-5-phosphate (active vitamin B6) as a cofactor. This completely bypasses tryptophan hydroxylase (TPH2), the rate-limiting enzyme in the normal serotonin synthesis pathway from dietary L-tryptophan. The result is a reliable, dose-dependent increase in serotonin across multiple brain regions including the dorsal raphe nucleus, hippocampus, and prefrontal cortex. Elevated serotonin activates 5-HT1A autoreceptors (calming), 5-HT2A/2C postsynaptic receptors (mood modulation), and 5-HT3 receptors (gut-brain signaling). In the pineal gland, serotonin is converted by arylalkylamine N-acetyltransferase (AANAT) to N-acetylserotonin, then by hydroxyindole O-methyltransferase (HIOMT) to melatonin — explaining the sleep-promoting effects.

Holy Basil (Tulsi)

Holy basil's adaptogenic effects come from multiple compounds: eugenol (anti-inflammatory via COX-2 and 5-LOX inhibition, TRPV1 modulation), ursolic acid (cortisol modulation via 11beta-HSD inhibition and glucocorticoid receptor modulation), rosmarinic acid (antioxidant via Nrf2/ARE pathway, anti-allergic via mast cell stabilization), and ocimumosides A and B (anti-stress via CRH and corticosterone reduction). It modulates the HPA axis, normalizing cortisol and corticosterone levels through hypothalamic and adrenal effects. Ursolic acid inhibits acetylcholinesterase (AChE), mildly increasing synaptic acetylcholine. Eugenol provides direct anxiolytic effects through GABA-A receptor positive allosteric modulation (possibly at the beta2/3 subunit interface) and 5-HT1A partial agonism. Ocimumosides may reduce ACTH release from the pituitary.

Risks & Safety

5-HTP

Common

Nausea, diarrhea, stomach cramps.

Serious

Serotonin syndrome when combined with SSRIs, SNRIs, MAOIs, or tramadol — DO NOT combine without medical supervision.

Rare

Eosinophilia-myalgia syndrome (historical concern from contaminated L-tryptophan, not confirmed with modern 5-HTP).

Holy Basil (Tulsi)

Common

Very well-tolerated. Mild blood sugar lowering.

Serious

May have anti-fertility effects — caution if trying to conceive. May interact with blood thinners.

Rare

Allergic reaction.

Full Profiles