Uridine

A nucleotide base that plays a critical role in brain cell membrane synthesis, synapse formation, and dopamine receptor expression. Part of the well-known 'Mr. Happy Stack' (Uridine + Fish Oil + Choline) that synergistically supports mood and cognitive function. Uridine monophosphate is the preferred supplemental form for brain bioavailability.

Dosage

Standard: 150-250 mg Uridine Monophosphate sublingually or orally, twice daily. Best combined with fish oil (DHA) and a choline source for the full 'Mr. Happy Stack' protocol.

Dosages shown are for research reference only. Always consult a qualified healthcare provider.

Half-Life

2-5 hours

Administration

Sublingual or oral. Uridine monophosphate is the preferred form. Triacetyluridine (TAU) is a more bioavailable prodrug.

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Mechanism of Action

Uridine (as UMP) is phosphorylated to UTP and enters the Kennedy pathway, where it combines with choline via CTP:phosphocholine cytidylyltransferase to form CDP-choline — the rate-limiting step in phosphatidylcholine synthesis. Uridine provides the nucleotide component needed for constructing phosphatidylcholine in neuronal cell membranes and synaptic vesicles. Uridine stimulates neurite outgrowth and synaptogenesis via activation of P2Y receptors and downstream PI3K/Akt signaling. It upregulates dopamine D2 receptor expression in the striatum and enhances dopaminergic neurotransmission. When combined with DHA (from fish oil) and choline, the three compounds synergistically increase synaptic membrane synthesis, dendritic spine density, and dopaminergic signaling — the 'Mr. Happy Stack' mechanism.

Regulatory Status

Dietary supplement. Available without prescription.

Risks & Safety

Common

Mild gastrointestinal discomfort, diarrhea.

Serious

Very limited long-term human supplementation data.

Rare

Lethargy, vivid dreams, depressive symptoms in some users.

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Research Papers

10
[Effect of uridine on mitochondrial function].

Published: September 24, 2023

AI Summary

Furthermore, it can protect cells from hypoxic injury by reducing intracellular oxidative stress, promoting high-energy compounds synthesis. Previous studies have shown that the protective effects of uridine are closely related to its effect on mitochondria.

Uridine natural products: Challenging targets and inspiration for novel small molecule inhibitors.

Published: September 14, 2020

AI Summary

This research has culminated in the development of complementary strategies that can lead to effective uridine-based inhibitors and antibiotics. The strengths and challenges of the juxtaposing methods will be illustrated by examining select uridine natural products.

Uridine and its role in metabolic diseases, tumors, and neurodegenerative diseases.

Published: February 28, 2024

AI Summary

Abnormal levels of blood uridine have been found in people with diabetes and obesity, suggesting a link of uridine dysregulation and metabolic disorders. This article summarizes the latest findings about the metabolic effects of uridine and the potential of uridine metabolism as therapeutic target in treatment of metabolic disorders.

Uridine Metabolism and Its Role in Glucose, Lipid, and Amino Acid Homeostasis.

Published: April 13, 2020

AI Summary

Uridine is mostly present in blood and cerebrospinal fluid, where it contributes to the maintenance of basic cellular functions affected by UPase enzyme activity, feeding habits, and ATP depletion. This review is devoted to uridine metabolism and its role in glucose, lipid, and amino acid homeostasis.

Uridine receptor: discovery and its involvement in sleep mechanism.

Published: April 30, 2001

AI Summary

Especially, N3-phenacyluridine markedly enhanced pentobarbital- and diazepam-induced sleep by 6- and 70-fold, respectively. It is suggested that uridine is released from steps of nucleic acid-nucleic protein biosynthesis (catabolism), and reaches the binding sites in the areas of the brain which regulate natural sleep.

Biochemistry of uridine in plasma.

Published: September 17, 2011

AI Summary

Factors associated with an increase in plasma concentration of uridine include enhanced ATP consumption, enhanced uridine diphosphate (UDP)-glucose consumption via glycogenesis, inhibited uridine uptake by cells via the nucleoside transport pathway, increased intestinal absorption, and increased 5-phosphribosyl-1-pyrophosphate and urea synthesis...

Uridine and its nucleotides: biological actions, therapeutic potentials.

Published: May 3, 1999

AI Summary

An understanding of how uridine and its nucleotides modulate such vastly complicated biological systems should ultimately lead to the development of new ways for modulating human physiology in both normal and diseased states.

The Role of Uridine in Health and Disease.

Published: July 28, 2025

AI Summary

Uridine plays a major role as a key biomolecule in health maintenance and disease treatment. The review aims to provide new perspectives and implications for uridine research and to promote further application of uridine in the medical field.

Uridine phosophorylase: an important enzyme in pyrimidine metabolism and fluoropyrimidine activation.

Published: May 20, 2004

AI Summary

In this review, we discuss in detail the role of UPase in the regulation of uridine homeostasis and pyrimidine metabolism and in the activation of fluoropyrimidines. To address its potential in cancer treatment, we will also discuss the regulatory mechanisms of UPase gene expression and its induction in tumor tissues.

Effects of uridine and nucleotides on hemostasis parameters.

Published: May 23, 2023

AI Summary

Our results showed that UDP, and especially UTP, inhibited ADP- and collagen-induced aggregation in a concentration-dependent manner. Collectively, our data showed that UTP inhibited platelet aggregation in a concentration-dependent manner and suppressed clot formation.

Frequently Asked Questions

What is Uridine used for?

A nucleotide base that plays a critical role in brain cell membrane synthesis, synapse formation, and dopamine receptor expression. Part of the well-known 'Mr. Happy Stack' (Uridine + Fish Oil + Choline) that synergistically supports mood and cognitive function. Uridine monophosphate is the preferred supplemental form for brain bioavailability.

What are the side effects of Uridine?

Common: Mild gastrointestinal discomfort, diarrhea. Serious: Very limited long-term human supplementation data. Rare: Lethargy, vivid dreams, depressive symptoms in some users.

How is Uridine administered?

Uridine is administered via sublingual or oral. uridine monophosphate is the preferred form. triacetyluridine (tau) is a more bioavailable prodrug..

What is the half-life of Uridine?

The half-life of Uridine is 2-5 hours.

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